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crispr guide verification tool  (Synthego Inc)

 
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    Synthego Inc crispr guide verification tool
    Fig. 2 A <t>CRISPR-Cas9-induced</t> t(4;11)/MLL-AF4 translocation in human FL HSPCs causes a dramatic increase in B-lineage cell proliferation in vitro. a Cumulative absolute number of human CD45+CD19+ cells per well over time during CRISPRMLL-AF4+ and control MS-5 coculture (n = 3; donors 1–3). ***p < 0.005 (two-way ANOVA with Sidak correction for multiple comparisons). Data are shown as mean ± SEM. Source data are provided as a Source Data file. b RT-qPCR of human CD45+ cells showing expression of MLL-AF4 and AF4-MLL (n = 3; donors 1–3) relative to GAPDH at week 4 of MS-5 coculture. Data are shown as mean ± SEM. Source data are provided as a Source Data file. c. Representative flow cytometry plots of viable, single cells from control and CRISPRMLL-AF4+ cultures on week 4 of coculture. Custom lineage cocktail (Lin) = CD2/CD3/CD14/CD16/CD56/CD235a (Supplementary Table 4). d Quantification of human cell immunophenotypes as a percentage of human CD45+ (hCD45+) cells (left), and progenitor immunophenotypes as a percentage of hCD45+Lin−CD34+ cells (right), in control (n = 3; donors 1–3) and CRISPRMLL-AF4+ (n = 3; donors 1–3) cultures over time. X = data not shown because absolute number of hCD45+ cells is <50. Data are shown as mean ± SEM. Source data are provided as a Source Data file.
    Crispr Guide Verification Tool, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/crispr+guide+verification+tool/guide+tool+verification/pm34824279-224-15-19
    Average 86 stars, based on 1 article reviews
    crispr guide verification tool - by Bioz Stars, 2026-08
    86/100 stars

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    1) Product Images from "A human fetal liver-derived infant MLL-AF4 acute lymphoblastic leukemia model reveals a distinct fetal gene expression program."

    Article Title: A human fetal liver-derived infant MLL-AF4 acute lymphoblastic leukemia model reveals a distinct fetal gene expression program.

    Journal: Nature communications

    doi: 10.1038/s41467-021-27270-z

    Fig. 2 A CRISPR-Cas9-induced t(4;11)/MLL-AF4 translocation in human FL HSPCs causes a dramatic increase in B-lineage cell proliferation in vitro. a Cumulative absolute number of human CD45+CD19+ cells per well over time during CRISPRMLL-AF4+ and control MS-5 coculture (n = 3; donors 1–3). ***p < 0.005 (two-way ANOVA with Sidak correction for multiple comparisons). Data are shown as mean ± SEM. Source data are provided as a Source Data file. b RT-qPCR of human CD45+ cells showing expression of MLL-AF4 and AF4-MLL (n = 3; donors 1–3) relative to GAPDH at week 4 of MS-5 coculture. Data are shown as mean ± SEM. Source data are provided as a Source Data file. c. Representative flow cytometry plots of viable, single cells from control and CRISPRMLL-AF4+ cultures on week 4 of coculture. Custom lineage cocktail (Lin) = CD2/CD3/CD14/CD16/CD56/CD235a (Supplementary Table 4). d Quantification of human cell immunophenotypes as a percentage of human CD45+ (hCD45+) cells (left), and progenitor immunophenotypes as a percentage of hCD45+Lin−CD34+ cells (right), in control (n = 3; donors 1–3) and CRISPRMLL-AF4+ (n = 3; donors 1–3) cultures over time. X = data not shown because absolute number of hCD45+ cells is <50. Data are shown as mean ± SEM. Source data are provided as a Source Data file.
    Figure Legend Snippet: Fig. 2 A CRISPR-Cas9-induced t(4;11)/MLL-AF4 translocation in human FL HSPCs causes a dramatic increase in B-lineage cell proliferation in vitro. a Cumulative absolute number of human CD45+CD19+ cells per well over time during CRISPRMLL-AF4+ and control MS-5 coculture (n = 3; donors 1–3). ***p < 0.005 (two-way ANOVA with Sidak correction for multiple comparisons). Data are shown as mean ± SEM. Source data are provided as a Source Data file. b RT-qPCR of human CD45+ cells showing expression of MLL-AF4 and AF4-MLL (n = 3; donors 1–3) relative to GAPDH at week 4 of MS-5 coculture. Data are shown as mean ± SEM. Source data are provided as a Source Data file. c. Representative flow cytometry plots of viable, single cells from control and CRISPRMLL-AF4+ cultures on week 4 of coculture. Custom lineage cocktail (Lin) = CD2/CD3/CD14/CD16/CD56/CD235a (Supplementary Table 4). d Quantification of human cell immunophenotypes as a percentage of human CD45+ (hCD45+) cells (left), and progenitor immunophenotypes as a percentage of hCD45+Lin−CD34+ cells (right), in control (n = 3; donors 1–3) and CRISPRMLL-AF4+ (n = 3; donors 1–3) cultures over time. X = data not shown because absolute number of hCD45+ cells is <50. Data are shown as mean ± SEM. Source data are provided as a Source Data file.

    Techniques Used: CRISPR, Translocation Assay, In Vitro, Control, Quantitative RT-PCR, Expressing, Cytometry



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    crispr guide verification tool  (Synthego Inc)
    86
    Synthego Inc crispr guide verification tool
    Fig. 2 A <t>CRISPR-Cas9-induced</t> t(4;11)/MLL-AF4 translocation in human FL HSPCs causes a dramatic increase in B-lineage cell proliferation in vitro. a Cumulative absolute number of human CD45+CD19+ cells per well over time during CRISPRMLL-AF4+ and control MS-5 coculture (n = 3; donors 1–3). ***p < 0.005 (two-way ANOVA with Sidak correction for multiple comparisons). Data are shown as mean ± SEM. Source data are provided as a Source Data file. b RT-qPCR of human CD45+ cells showing expression of MLL-AF4 and AF4-MLL (n = 3; donors 1–3) relative to GAPDH at week 4 of MS-5 coculture. Data are shown as mean ± SEM. Source data are provided as a Source Data file. c. Representative flow cytometry plots of viable, single cells from control and CRISPRMLL-AF4+ cultures on week 4 of coculture. Custom lineage cocktail (Lin) = CD2/CD3/CD14/CD16/CD56/CD235a (Supplementary Table 4). d Quantification of human cell immunophenotypes as a percentage of human CD45+ (hCD45+) cells (left), and progenitor immunophenotypes as a percentage of hCD45+Lin−CD34+ cells (right), in control (n = 3; donors 1–3) and CRISPRMLL-AF4+ (n = 3; donors 1–3) cultures over time. X = data not shown because absolute number of hCD45+ cells is <50. Data are shown as mean ± SEM. Source data are provided as a Source Data file.
    Crispr Guide Verification Tool, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/crispr+guide+verification+tool/guide+tool+verification/pm34824279-224-15-19
    Average 86 stars, based on 1 article reviews
    crispr guide verification tool - by Bioz Stars, 2026-08
    86/100 stars
    		  Buy from Supplier

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    Fig. 2 A CRISPR-Cas9-induced t(4;11)/MLL-AF4 translocation in human FL HSPCs causes a dramatic increase in B-lineage cell proliferation in vitro. a Cumulative absolute number of human CD45+CD19+ cells per well over time during CRISPRMLL-AF4+ and control MS-5 coculture (n = 3; donors 1–3). ***p < 0.005 (two-way ANOVA with Sidak correction for multiple comparisons). Data are shown as mean ± SEM. Source data are provided as a Source Data file. b RT-qPCR of human CD45+ cells showing expression of MLL-AF4 and AF4-MLL (n = 3; donors 1–3) relative to GAPDH at week 4 of MS-5 coculture. Data are shown as mean ± SEM. Source data are provided as a Source Data file. c. Representative flow cytometry plots of viable, single cells from control and CRISPRMLL-AF4+ cultures on week 4 of coculture. Custom lineage cocktail (Lin) = CD2/CD3/CD14/CD16/CD56/CD235a (Supplementary Table 4). d Quantification of human cell immunophenotypes as a percentage of human CD45+ (hCD45+) cells (left), and progenitor immunophenotypes as a percentage of hCD45+Lin−CD34+ cells (right), in control (n = 3; donors 1–3) and CRISPRMLL-AF4+ (n = 3; donors 1–3) cultures over time. X = data not shown because absolute number of hCD45+ cells is <50. Data are shown as mean ± SEM. Source data are provided as a Source Data file.

    Journal: Nature communications

    Article Title: A human fetal liver-derived infant MLL-AF4 acute lymphoblastic leukemia model reveals a distinct fetal gene expression program.

    doi: 10.1038/s41467-021-27270-z

    Figure Lengend Snippet: Fig. 2 A CRISPR-Cas9-induced t(4;11)/MLL-AF4 translocation in human FL HSPCs causes a dramatic increase in B-lineage cell proliferation in vitro. a Cumulative absolute number of human CD45+CD19+ cells per well over time during CRISPRMLL-AF4+ and control MS-5 coculture (n = 3; donors 1–3). ***p < 0.005 (two-way ANOVA with Sidak correction for multiple comparisons). Data are shown as mean ± SEM. Source data are provided as a Source Data file. b RT-qPCR of human CD45+ cells showing expression of MLL-AF4 and AF4-MLL (n = 3; donors 1–3) relative to GAPDH at week 4 of MS-5 coculture. Data are shown as mean ± SEM. Source data are provided as a Source Data file. c. Representative flow cytometry plots of viable, single cells from control and CRISPRMLL-AF4+ cultures on week 4 of coculture. Custom lineage cocktail (Lin) = CD2/CD3/CD14/CD16/CD56/CD235a (Supplementary Table 4). d Quantification of human cell immunophenotypes as a percentage of human CD45+ (hCD45+) cells (left), and progenitor immunophenotypes as a percentage of hCD45+Lin−CD34+ cells (right), in control (n = 3; donors 1–3) and CRISPRMLL-AF4+ (n = 3; donors 1–3) cultures over time. X = data not shown because absolute number of hCD45+ cells is <50. Data are shown as mean ± SEM. Source data are provided as a Source Data file.

    Article Snippet: Potential off-target editing sites for both MLL and AF4 sgRNAs were predicted using the Synthego CRISPR guide verification tool (https://design.synthego.com/#/validate).

    Techniques: CRISPR, Translocation Assay, In Vitro, Control, Quantitative RT-PCR, Expressing, Cytometry